Micronuclei frequency and exposure to chemical mixtures in three Colombian mining populations.

The Colombian mining industry has witnessed significant growth. Depending on the scale and mineral extracted, complex chemical mixtures are generated, impacting the health of occupationally exposed populations and communities near mining projects. Increasing evidence suggests that chromosomal instability (CIN) is an important link between the development of certain diseases and exposure to complex mixtures. To better understand the effects of exposure to complex mixtures we performed a biomonitoring study on 407 healthy individuals from four areas: three located in municipalities exploiting different-scale mining systems and a reference area with no mining activity. Large, medium, and small-scale mining systems were analyzed in Montelibano (Córdoba), artisanal and small-scale mining (ASGM) in Nechí (Antioquia), and a closed mining system in Aranzazu (Caldas). The reference area with no mining activity was established in Montería (Córdoba). ICP-MS measured multi-elemental exposure in hair, and CIN was evaluated using the cytokinesis-block micronucleus technique (MNBN). Exposure to mixtures of chemical elements was comparable in workers and residents of the mining areas but significantly higher compared to reference individuals. In Montelibano, increased MNBN frequencies were associated with combined exposure to Se, Hg, Mn, Pb, and Mg. This distinct pattern significantly differed from other areas. Specifically, in Nechí, Cr, Ni, Hg, Se, and Mg emerged as the primary contributors to elevated frequencies of MNBN. In contrast, a combination of Hg and Ni played a role in increasing MNBN in Aranzazu. Interestingly, Se consistently correlated with increased MNBN frequencies across all active mining areas. Chemical elements in Montelibano exhibit a broader range compared to other mining zones, reflecting the characteristics of the high-impact and large-scale mining in the area. This research provides valuable insights into the effects of exposure to chemical mixtures, underscoring the importance of employing this approach in the risk assessment of communities, especially those from residential areas.

Genetic Instability among Hitnü People Living in Colombian Crude-Oil Exploitation Areas.

Oil exploitation, drilling, transportation, and processing in refineries produces a complex mixture of chemical compounds, including polycyclic aromatic hydrocarbons (PAHs), which may affect the health of populations living in the zone of influence of mining activities (PZOI). Thus, to better understand the effects of oil exploitation activities on cytogenetic endpoint frequency, we conducted a biomonitoring study in the Hitnü indigenous populations from eastern Colombia by using the cytokinesis micronucleus cytome assay (CBMN-cyt). PAH exposure was also measured by determine urine 1-hydroxypyrene (1-OHP) using HPLC. We also evaluated the relationship between DNA damage and 1-OHP levels in the oil exploitation area, as well as the modulating effects of community health factors, such as Chagas infection; nutritional status; and consumption of traditional hallucinogens, tobacco, and wine from traditional palms. The frequencies of the CBMN-cyt assay parameters were comparable between PZOI and Hitnü populations outside the zone of influence of mining activities (POZOI); however, a non-significant incremental trend among individuals from the PZOI for most of the DNA damage parameters was also observed. In agreement with these observations, levels of 1-OHP were also identified as a risk factor for increased MN frequency (PR = 1.20) compared to POZOI (PR = 0.7). Proximity to oil exploitation areas also constituted a risk factor for elevated frequencies of nucleoplasmic bridges (NPBs) and APOP-type cell death. Our results suggest that genetic instability and its potential effects among Hitnü individuals from PZOI and POZOI could be modulated by the combination of multiple factors, including the levels of 1-OHP in urine, malnutrition, and some traditional consumption practices.

IL-4, IL-10, CCL2 and TGF-ß as potential biomarkers for severity in Plasmodium vivax malaria.

Cytokines and chemokines are immune response molecules that display diverse functions, such as inflammation and immune regulation. In Plasmodium vivax infections, the uncontrolled production of these molecules is thought to contribute to pathogenesis and has been proposed as a possible predictor for disease complications. The objective of this study was to evaluate the cytokine profile of P. vivax malaria patients with different clinical outcomes to identify possible immune biomarkers for severe P. vivax malaria. The study included patients with non-severe (n = 56), or severe (n = 50) P. vivax malaria and healthy controls (n = 50). Patient plasma concentrations of IL-4, IL-2, CXCL10, IL-1ß, TNF-α, CCL2, IL-17A, IL-6, IL-10, IFN-γ, IL-12p70, CXCL8 and active TGF-ß1 were determined through flow cytometry. The levels of several cytokines and chemokines, CXCL10, IL-10, IL-6, IL-4, CCL2 and IFN-γ were found to be significantly higher in severe, compared to non-severe P. vivax malaria patients. Severe thrombocytopenia was positively correlated with IL-4, CXCL10, IL-6, IL-10 and IFN-γ levels, renal dysfunction was related to an increase in IL-2, IL-1ß, IL-17A and IL-8, and hepatic impairment with CXCL10, MCP-1, IL-6 and IFN-γ. A Lasso regression model suggests that IL-4, IL-10, CCL2 and TGF-ß might be developed as biomarkers for severity in P. vivax malaria. Severe P. vivax malaria patients present specific cytokine and chemokine profiles that are different from non-severe patients and that could potentially be developed as biomarkers for disease severity.

Factores ambientales en la transmisión del SARS-CoV-2/COVID 19: panorama mundial y colombiano / Environmental factors in the transmission of SARS-COV-2/COVID 19: World and Colombian outlook

Resumen Introducción: Diversas investigaciones han intentado establecer el impacto de algunos parámetros meteorológicos y de calidad del medio ambiente en la transmisión del SARS-CoV-2, tomando en consideración las características geográficas de cada país y con el fin de mitigar el avance de la enfermedad mediante el control de esos factores. Objetivo: Analizar la evidencia existente sobre la posible relación entre factores ambientales y la morbilidad y mortalidad por SARS-CoV-2/COVID-19 en el panorama mundial y colombiano. Metodología: Se realizó una revisión exhaustiva de la literatura científica en las bases de datos electrónicas. Además, se analizó el impacto de algunas variables ambientales y la gravedad de los casos de COVID-19 durante el período del 8 de abril al 29 de julio de 2020 en la ciudad Bogotá. Resultados: El análisis correlacional entre la ocupación de camas UCIs en Bogotá con los factores ambientales como temperatura, las concentraciones de PM2 5, O3, NO, NO2 y CO mostraron una relación inversamente significativa. Entre tanto, se presentó una correlación positiva entre los niveles de óxidos de nitrógeno (NO/NO2) y el monóxido de carbono (CO). Algunos de estos resultados posiblemente están relacionados con los efectos de la cuarentena impuesta por el gobierno local. Conclusión: Nivel mundial existe suficiente evidencia para relacionar algunas condiciones y parámetros ambientales con un aumento en la morbilidad y mortalidad por COVID-19. Las evidencias a nivel nacional aún son escasas.

Abstract Introduction: Several investigations have attempted to establish the impact of some meteorological and environmental parameters on the transmission of SARS-CoV-2, considering each country's geographical characteristics and seeking to mitigate the disease's advancement by controlling these factors. Objective: Analyze the evidence on the possible relationship between environmental factors, morbidity, and mortality due to SARS-CoV-2/COVID-19, both globally and within Colombia. Methodology: A comprehensive review of the scientific literature was carried out in the electronic databases. Additionally, the impact of some environmental variables and the severity of COVID-19 cases were analyzed during the period from April 8 to July 29, 2020, for the city of Bogotá. Results: The correlational analysis between the ICU admission rates in Bogotá and the environmental factors like temperature, PM2 5, O3, NO, NO2 y CO levels, and ozone concentration showed an inversely significant relationship. Meanwhile, there was a positive correlation between the levels of nitrogen oxides (NO/NO2) and carbon monoxide (CO). Some of these results could be related to the effects of the quarantine imposed by local governments. Conclusion: Globally, there is enough evidence to link environmental conditions and parameters with increased morbidity and mortality for COVID-19. Evidence at the national level is still scarce.

Liver and kidney dysfunction, hypoglycemia, and thrombocytopenia in Plasmodium vivax malaria patients at a Colombian Northwest region.

Plasmodium vivax has high morbidity, it is the Plasmodium species with the greatest worldwide distribution, and its ability to trigger severe symptoms is currently recognized. The present study aims to compare the clinical and epidemiological characteristics of patients with P. vivax malaria, with and without complication criteria, in an endemic area for malaria transmission in northwest Colombia. A descriptive cross-sectional study was carried out between 2017 and 2019, patients with P.vivax severe malaria (n = 50), non-severe malaria (n = 56) and healthy controls (n = 50) were included. Sociodemographic, clinical, hematological, and biochemical characteristics were analyzed. Clinical follow-up was carried out in a group of patients with severe malaria. The statistical analysis was carried out in GraphPad Prism; the Chi-square test analyzed categorical variables, comparisons of variables for the three groups were carried out by the Kruskal-Wallis test and comparison between two groups by the Mann-Whitney test. A multiple correspondence analysis described the relationship between variables, which was carried out through the R software. One hundred fifty-six individuals were linked to the study, 76 women and 80 men, between 3 and 71 years old. For 50% of the patients, it was their first malaria episode; 42% of the patients classified with severe malaria required hospitalization, compared to 7.1% of the patients with non-severe malaria. Parasitaemia was similar in both clinical groups; however, 10% of severe patients presented high parasitemia, between 20,000-135,000. The most frequent clinical characteristics in patients with severe malaria were severe thrombocytopenia in 54%, hypoglycemia in 48%, and liver and kidney failure in 30%. Biochemical and hematological parameters returned to normal in 90% of the patients with severe malaria on the third day after starting treatment. Thrombocytopenia, hypoglycemia, and liver and kidney dysfunctions were the most frequent P. vivax malaria complications in this study. Hemoglobin concentration and parasite count were not related to the clinical condition of patients. Thrombocytopenia was the most frequent finding in patients with malaria, and its severity presented an inverse relationship with the number of previous malaria episodes.

DENV and ZIKV detection in patients with acute febrile syndrome in Córdoba, Colombia.

OBJECTIVE: To determine simultaneous circulation of DENV serotypes and ZIKV in Córdoba, Colombia, during 2015 and 2016. MATERIAL AND METHODS: A total of 294 samples from patients with clinical diagnosis of febrile syndrome compatible with dengue were collected between June 2015 and December 2016. All samples were tested for DENV and ZIKV by RT-PCR using C6/36 cells culture supernatant. RESULTS: Thirty-three percent of the samples were positive (97/294); from these, 61.8% were positive for DENV and 31% were positive for Zika. The predominant serotype was DENV-2 (70.1%), followed by DENV-3 (8.9%), DENV-4 (6%), and DENV-1 (3%). DENV/ZIKV coinfection was identified in 7.2% of the cases associated with DENV-1 and DENV-3 serotypes. The confirmed cases of dengue, Zika, and DENV/ZIKV coinfections were clinically mild and self-limited. CONCLUSIONS: We reported the co-circulation of all four DENV serotypes, with a higher frequency of DENV-2, and ZIKV introduction in Córdoba department-Colombia in August 2015. This scenario favored the appearance of DENV/ZIKV coinfections.

Nuclear factor erythroid 2 - related factor 2 and its relationship with cellular response in nickel exposure: a systems biology analysis.

BACKGROUND: Nickel and nickel-containing compounds (NCC) are known human carcinogens. However, the precise molecular mechanisms of nickel-induced malignant transformation remain unknown. Proposed mechanisms suggest that nickel and NCC may participate in the dual activation/inactivation of enzymatic pathways involved in cell defenses against oxidative damage, where Nuclear factor-erythroid 2 related factor 2 (Nrf2) plays a central role. METHODS: For assessing the potential role of proteins involved in the Nrf2-mediated response to nickel and NCC exposure, we designed an interactome network using the STITCH search engine version 5.0 and the STRING software 10.0. The major NCC-protein interactome (NCPI) generated was analyzed using the MCODE plugin, version 1.5.1 for the detection of interaction modules or subnetworks. Main centralities of the NCPI were determined with the CentiScape 2.2 plugin of Cytoscape 3.4.0 and main biological processes associated with each cluster were assessed using the BiNGO plugin of Cytoscape 3.4.0. RESULTS: Water-soluble NiSO4 and insoluble Ni3S2 were the most connected to proteins involved in the NCPI network. Nfr2 was detected as one of the most relevant proteins in the network, participating in several multifunctional protein complexes in clusters 1, 2, 3 and 5. Ontological analysis of cluster 3 revealed several processes related to unfolded protein response (UPR) and response to endoplasmic reticulum (ER) stress. CONCLUSIONS: Cellular response to NCC exposure was very comparable, particularly concerning oxidative stress response, inflammation, cell cycle/proliferation, and apoptosis. In this cellular response, Nfr2 was highly centralized and participated in several multifunctional protein complexes, including several related to ER-stress. These results add evidence on the possible Ni2+ induced - ER stress mainly associated with insoluble NCC. In this scenario, we also show how protein degradation mediated by ubiquitination seems to play key roles in cellular responses to Ni.

New Scenarios of Chagas Disease Transmission in Northern Colombia.

Chagas disease (CD) is a systemic parasitic infection caused by the flagellated form of Trypanosoma cruzi. Córdoba department, located in the Colombian Caribbean Coast, was not considered as a region at risk of T. cruzi transmission. In this article, we describe the first acute CD case in Salitral village in Sahagún, Córdoba, confirmed by microscopy and serological tests. Our results draw attention to a new scenario of transmission of acute CD in nonendemic areas of Colombia and highlight the need to include CD in the differential diagnosis of febrile syndromes in this region.

Colonización y factores de virulencia de Staphylococcus aureus resistente a meticilina en una población infantil de Montería / Colonization and virulence factors of methicillin resistant Staphylococcus aureus in a pediatric population in Montería, Colombia / Colonização e fatores de virulência de Staphylococcus aureus resistente a meticilina numa população infantil de Montería

Introducción: Staphylococcus aureus resistente a meticilina (SARM) puede colonizar el cuerpo humano, con mayor frecuencia en las fosas nasales, pero también en las manos, el periné y la faringe. Además, se ha propuesto que la colonización puede ser un factor de riesgo para adquirir infecciones futuras. Objetivo: determinar la prevalencia y las características microbiológicas y moleculares del SARM en una población infantil sana. Metodología: se hizo un estudio descriptivo transversal para determinar la tasa de colonización nasal por SARM en 150 niños pertenecientes a 13 hogares infantiles de la ciudad de Montería. Los aislamientos se hicieron a partir de hisopados nasales y faríngeos, se identificaron mediante pruebas microbiológicas convencionales y se confirmaron y caracterizaron por métodos moleculares. Resultados: la tasa de colonización por SARM fue del 9,3% (14/150). El 62,5% de los aislamientos portaban el SCCmec subtipo IVc; 87,5% de los aislamientos presentaron los genes que codifican para PVL y Sek, mientras que 81,2% portaban el gen bsaB. Conclusión: el porcentaje de colonización hallado es uno de los más altos reportados para la población infantil de la región Caribe colombiana, y los aislamientos presentaron factores de virulencia relacionados con cuadros clínicos agresivos.

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is able to colonize the human body, most frequently the nostrils, but also the hands, perineum and throat. Such colonization has been proposed as a risk factor to acquire future infections. Objective: To determine the prevalence, and the microbiological and molecular characteristics of MRSA in healthy children. Methodology: A cross-sectional descriptive study was done of 150 children from 13 day care centers in Montería, Colombia. Nasal and throat swabs were obtained. The isolates were identified and characterized by microbiological and molecular methods. Results: The MRSA colonization rate was 9.3% (14/150). 62.5% of the isolates carried the subtype IVc of SCCmec, and 87.5% had the genes encoding for PVL and Sek, while 81.2% carried the gene bsaB. Conclusion: The percentage of colonization found is one of the highest reported among children from the Colombian Caribbean region, and the isolates have virulence factors that have been associated with an aggressive clinical course.

Introdução: Staphylococcus aureus resistente a meticilina (SARM) pode colonizar o corpo humano, com maior frequência nas fossas nasais, mas também nas mãos, o períneo e a faringe. Ademais, propôs-se do que a colonização pode ser um fator de risco para adquirir infecções futuras. Objetivo: determinar a prevalência e as características microbiológicas e moleculares do SARM numa população infantil sã. Metodologia: fez-se um estudo descritivo transversal para determinar a taxa de colonização nasal por SARM em 150 crianças pertencentes a 13 lares infantis da cidade de Montería. Os isolamentos se fizeram a partir de amostras com suabe nasais e faríngeos, identificaram-se mediante provas microbiológicas convencionais e se confirmaram e caracterizaram por métodos moleculares. Resultados: a taxa de colonização por SARM foi de 9,3 % (14/150). 62,5% dos isolamentos portavam o SCCmec subtipo IVc; 87,5% dos isolamentos apresentaram os genes que codificam para PVL e Sek, enquanto 81,2% portavam o gene bsaB. Conclusão: a porcentagem de colonização achado é um dos mais altos reportados para a população infantil da região Caribe colombiana, e os isolamentos apresentaram fatores de virulência relacionados com quadros clínicos agressivos.